A novel preparation method of smart responsive glass sphere based composite carriers was reported in this study. Firstly, random block copolymers were synthesized by free radical polymerization of N⁃isopropylacrylamide, hydroxypropyl methacrylate and 3⁃trimethoxysilypropyl methacrylate. Then, the copolymer solution was uniformly sprayed on the surfaces of glass spheres by means of a self⁃made bottom spray fluidized bed reactor, and the bonding between copolymers and glass spheres was fabricated by thermal annealing, so as to form the copolymer/glass sphere composite carriers. The coating effects of the smart copolymers on the surfaces of glass spheres were investigated, including characteristic functional groups, surface microstructure, biocompatibility and thermosensibility. The results show that the temperature⁃responsive copolymers can be linked to the surfaces of glass spheres by bottom⁃spray coating technology, and the copolymer layers with certain thickness can be formed on the sphere surfaces. The composite carriers have good biocompatibility, and can realize effective cell adhesion and spontaneous desorption by using its intelligent response characteristics.